产品说明
一般描述
The widely used MMP substrate FS-1 (Cat. No. 03-32-5032), modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and sensitivity. Exhibits a 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 (Km (μM)= 27.5, 5.2, and 7.9, respectively) when compared with FS-1 and ~ 2-fold higher kcat/Km (778, 400 M-1 s-1) towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10. Excitation max: 324 nm; Emission max: 400 nm.
The widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher kcat/Km towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.
包装
1 mg in Glass bottle
Packaged under inert gas
生化/生理作用
Product does not compete with ATP.
Reversible: no
Primary Target
Broad-spectrum MMP and TACE substrate
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Neumann, U., et al. 2004. Anal. Biochem.328, 166.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C55H80N16O16 |
分子量 | 1221.32 |
产品性质
质量水平 | 100 |
测定 | ≥97% (HPLC) |
形式 | lyophilized |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
溶解性 | water: 20 mg/mL |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich