产品说明
一般描述
An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.
An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (kcat = 21.6 s-1 when the substrate concentration is half the Vmax, S0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.
包装
1 mg in Glass bottle
生化/生理作用
Product does not compete with ATP.
Primary Target
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity
kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Jin, G., et al. 2002. Anal. Biochem.302, 269.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C59H93N23O20 |
分子量 | 1444.51 |
产品性质
质量水平 | 100 |
测定 | ≥95% (HPLC) |
形式 | lyophilized solid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze desiccated protect from light |
颜色 | yellow |
溶解性 | DMSO: 5 mg/mL |
荧光 | λex ~320 nm λem ~420 nm |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich