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位置:首页 > 品牌 > Sigma-Aldrich > CHO-K1.Cl-0236 / CHO-K1.Cl-0239

CHO-K1.Cl-0236 / CHO-K1.Cl-0239

货号
CB_15042909
规格纯度
from hamster ovary, 15042909
参考价格
10565.5 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
CHO-K1.Cl-0236 / CHO-K1.Cl-0239
产品介绍:

产品说明

细胞系来源

Stably transfected with a pcDNA3.1/Hygro(+) based vector with a cDNA insert that had been modified to enable expression of Red-crowned Mangabey / Olive baboon FcγRIIIa as a GPI linked receptor

细胞系描述

Clone of CHO-K1 cells expressing Cercocebus torquatus (Red-crowned Mangabey) / *Papio anubis (Olive baboon) FcγRIIIa anchored to glycosyl phosphatidylinositol.
* Sequences predicted from NCBI DQ423376.1 and NCBI NM_001112647.1, respectively, are identical at the amino acid level

培养基

MEM (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 10% FBS (F2442) with 600ug/ml hygromycin B

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO2/95%air; 37℃

其他说明

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

引用

If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.Cl-0236 / CHO-K1.Cl-0239 (ECACC 15042909)

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

产品性质

生物来源hamster ovary
描述CHO-K1, non-human primate FcγRIIIa, Fc gamma receptor 3A, Cercocebus torquatus, red-crowned mangabey, papio anubis, olive baboon
生长模式Adherent
形态学Polygonal
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

Sigma-Aldrich

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