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Atto 490LS maleimide

品牌
Sigma-Aldrich
货号
78363
规格纯度
suitable for fluorescence
参考价格
4396.66 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Atto 490LS maleimide
产品介绍:

产品说明

一般描述

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.
Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

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法律信息

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

基本信息

NACRESNA.32

产品性质

分子量Mw 818 g/mol
质量水平100
荧光λex 496 nm; λem 640.0 nm±10 nm in PBS, pH 7.4
适用性suitable for fluorescence
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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