产品说明
一般描述
膜蛋白占据了生物体基因组大约20-30%的成分，充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能，例如将细胞与外部的毒素隔离开来，作为胞内信号级联的起始点，以及维持临界离子浓度。
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
应用
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
适用性
提供足够进行80次检测的试剂。
法律信息
CelLytic is a trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.56 |
产品性质
| 质量水平 | 200 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
组份列表
组份不可单独销售
| 货号 | 组份 |
| Lysis and Separation Buffer 50 mL | |
| Wash Buffer for CelLytic MEM 50 mL | |
| Sodium Chloride, 4M Solution 1.5 mL |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | 188.6 °F - closed cup |
| 闪点(C) | 87 ℃ - closed cup |
Sigma-Aldrich
m.cnreagent.com
