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Anti-Leukemia Inhibitory Factor antibody produced in goat

品牌
Sigma-Aldrich
货号
L9152
规格纯度
IgG fraction of antiserum, lyophilized powder
参考价格
7088.48 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Leukemia Inhibitory Factor antibody produced in goat
Emfilermin
D factor
LIF
CDF
differentiation inhibitory activity
DIA
产品介绍:

产品说明

特异性

The antibody neutralizes the biological activity of recombinant mouse LIF and also neutralizes the biologicl activity of recombinant human LIF at a 10-fold higher IgG concentration. Based on ELISA and immunoblotting, this antibody shows <10% cross-reactiity with recombinant human LIF.

免疫原

purified, E. coli-derived, recombinant mouse LIF.

应用

For Western blot a concentration of 0.1 μg/ml is recommended. For immunohistochemistry, a working dilution of 5-15 μg/ml is recommended. The neutralization dose is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.
Optimal dilutions should be determined by the end user.

生化/生理作用

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

制备说明

Protein A or G purified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00283185

产品性质

质量水平100
生物来源goat
偶联物unconjugated
抗体形式IgG fraction of antiserum
antibody product typeprimary antibodies
克隆polyclonal
形式lyophilized powder
species reactivitymouse
technique(s)immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
UniProt登记号P09056
储存温度−20℃
Gene Informationhuman ... LIF(3976)
mouse ... Lif(16878)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

Sigma-Aldrich

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