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Cathepsin D/E Substrate, Fluorogenic-Calbiochem

品牌
Sigma-Aldrich
货号
219360
规格纯度
An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L .
参考价格
4187.54 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Cathepsin D/E Substrate, Fluorogenic-Calbiochem
MOCAc-GKPILF~FRLK(Dnp)-D-R-NH2
MOCAc-GKPILF~FRLK(Dnp)- D-R-NH2
产品介绍:

产品说明

一般描述

An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.

包装

1 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Substrate for cathepsins D and E
kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively

警告

Toxicity: Standard Handling (A)

序列

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂

MOCAc = (7-Methoxycoumarin-4-yl)acetyl

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Yasuda, Y., et al. 1999. J. Biochem.126, 260.
Yasuda, Y., et al. 1999. J. Biochem.125, 1137.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C85H122N22O19
分子量1756.01

产品性质

质量水平100
测定≥97% (HPLC)
形式lyophilized solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
desiccated (hygroscopic)
protect from light
颜色 yellow
溶解性DMSO: 5 mg/mL
荧光λex ~328  nm
λem ~393 nm
运输ambient
储存温度2-8℃

Sigma-Aldrich

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