产品说明
一般描述
DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.
免疫原
synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.
应用
Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.
生化/生理作用
Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac®.
目标描述
DARPP32 phosphorylated on Thr75 acts as an inhibitor of PKA.
外形
Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
其他说明
The labeling by the antibody to DARPP32 Thr75 is markedly increased by okadaic acid treatment.
法律信息
Prozac is a registered trademark of Eli Lilly and Co.
基本信息
| MDL编号 | MFCD08705496 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous glycerol solution |
| 用途 | sufficient for 10 blots |
| 分子量 | antigen 32 kDa |
| species reactivity | mouse, human |
| technique(s) | dot blot: 1:1,000 western blot: 1:1,000 |
| UniProt登记号 | Q9UD71 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PPP1R1B(84152) mouse ... Ppp1r1b(19049) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
