产品说明

一般描述

Carboxylesterase 2 human gene is mapped to human chromosome 16q22.1.
Carboxylesterase belongs to the α/β hydrolase fold family and is involved in drug metabolism and activation. It is present in colon, liver, small intestine, heart, brain and testis. The hydrolase activity of small intestine is attributed to carboxylesterase 2 human. It has two glycosylation sites at 103 and 267 residues with molecular mass 60kDa.

应用

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.
Carboxylesterase 2 human has been used:

• in the in vitro enzyme-based inhibitor screening assay
• in substrate selectivity assay with emission ratiometric two-photon probe (SE1)
• as a reference standard in the enzyme assay with various 4-nitrophenyl and 1-naphthyl based substrates

Carboxylesterase 2 may play a key role in biotransformation of a variety of ester containing drugs and prodrugs.

生化/生理作用

Carboxylesterase 2 human identifies the site containing a large alcohol and small acyl group. It shows less transesterification activity due to presence of conformational interference in active site.
可水解酯和酰胺键的丝氨酸酯酶家族成员。羧基酯酶2是一种内质网结合的水解酶，在异源生物解毒和活化含酯治疗剂中起关键作用。羧基酯酶2也可参与海洛因和可卡因等药物的解毒过程。该酶被认为在脂质代谢中起作用。

单位定义

One unit will produce 1 nanomole of 4-nitrophenol from 4-nitrophenyl acetate per minute at pH 7.4 at 37 deg C.

基本信息

酶学委员会(EC)编号	3.1.1.1 ( BRENDA | IUBMB )
NACRES	NA.54

产品性质

质量水平	200
重组	expressed in baculovirus infected BTI insect cells
形式	liquid
specific activity	≥500 units/mg protein
分子量	60 kDa
重量	(0.5 ml)
运输	dry ice
储存温度	−70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable