产品说明
一般描述
Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
包装
1 g in Glass bottle
10 g in Plastic ampoule
生化/生理作用
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.
警告
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
重悬
Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.
其他说明
Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C18H16BrN5S |
| 分子量 | 414.32 |
| MDL编号 | MFCD00011964 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥98% (silver nitrate titration) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 杂质 | ≤0.5% Sulfated ash |
| 颜色 | yellow |
| 溶解性 | PBS: 2 mg/mL |
| 痕量阳离子 | heavy metals: ≤0.001% |
| 运输 | ambient |
| 储存温度 | 15-25℃ |
| InChI | 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1 |
| InChI key | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
安全信息
| 象形图 |   |
| 警示用语： | Warning |
| 危险声明 | H315 - H319 - H335 - H341 |
| 预防措施声明 | P201 - P202 - P261 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
