产品说明
一般描述
The Human Osteoprotegerin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Osteoprotegerin in serum, plasma, cell culture supernatants and urine.
Osteoprotegerin (OPG) is encoded by the gene mapped to human chromosome 8q24. It is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor kappa-B ligand) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). OPG is expressed in a wide variety of tissues, including adult heart, lung, kidney and liver. It is secreted both as a monomeric and a dimeric protein.
免疫原
Recombinant Human OPG
应用
请参考Protocol了解详情。
生化/生理作用
Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells. This process leads to excess accumulation of bone and cartilage. Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders(CVDs). It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma. OPG plays a vital role in bone metabolism and is involved in inhibition of ovariectomy-associated bone loss in rats. It is also used as a potent biomarker for CVD.
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
基本信息
| NACRES | NA.32 |
产品性质
| species reactivity | human |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable capture ELISA: suitable |
| input | sample type serum sample type plasma sample type urine sample type cell culture supernatant(s) |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 1.23-900 pg/mL |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TNFRSF11B(4982) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABELADA | ELISA 1X Assay/Sample Diluent Buffer A (Item D1) |
| RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
| RABSTOP3 | ELISA Stop Solution (Item I) |
| RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
| RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
Sigma-Aldrich
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