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IgM Isotype Control from murine myeloma

品牌
Sigma-Aldrich
货号
M5909
规格纯度
clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin
参考价格
1809.65 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
IgM Isotype Control from murine myeloma
Mouse IgM Isotype Control from myeloma
产品介绍:

产品说明

一般描述

Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.
The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.

应用

IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.
IgM isotype control antibodies are suitable for use in immunohistochemistry .
IgM Isotype Control from murine myeloma has been used:

  • in chromatin immunoprecipitation (ChIP) assay
  • LABScreen assays
  • immunofluorescent staining

原理

The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00164813
NACRESNA.46

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
克隆MOPC 104E, monoclonal
形式buffered aqueous solution
浓度200 μg/mL
technique(s)flow cytometry: suitable
同位素/亚型IgMλ
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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