Anti-Actin (Ab-1) Mouse mAb (JLA20)

产品说明

一般描述

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.

Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1

Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN

Entrez Gene Name: actin, alpha 1, skeletal muscle

Hu Entrez ID: 58, 71 (Related Antibodies: CP64)

Mu Entrez ID: 11459 ;-; 11465

Rat Entrez ID: 29437 ;-; 287876

Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.

免疫原

Chicken
chicken cytoskeletal proteins

包装

1 ea in Plastic ampoule

应用

Immunoblotting (1:5000-1:20000)

Immunofluorescence (1:10000, see application references)



Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

外形

100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

重悬

Following initial thaw, aliquot ascites and freeze (-20°C).

分析说明

Positive Control
All animal cell lines

其他说明

Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	JLA20, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)
species reactivity (predicted by homology)	all
manufacturer/tradename	Calbiochem®
储存条件	OK to freeze
同位素/亚型	IgM
运输	wet ice
储存温度	−20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable