您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-Actin (Ab-1) Mouse mAb (JLA20)

Anti-Actin (Ab-1) Mouse mAb (JLA20)

品牌
Sigma-Aldrich
货号
CP01
规格纯度
liquid, clone JLA20, Calbiochem®
参考价格
5854.74 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Actin (Ab-1) Mouse mAb (JLA20)
产品介绍:

产品说明

一般描述

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.

  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876

  • Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.

    免疫原

    Chicken
    chicken cytoskeletal proteins

    包装

    1 ea in Plastic ampoule

    应用

    Immunoblotting (1:5000-1:20000)

    Immunofluorescence (1:10000, see application references)



    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

    警告

    Toxicity: Multiple Toxicity Values, refer to MSDS (O)

    外形

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    重悬

    Following initial thaw, aliquot ascites and freeze (-20°C).

    分析说明

    Positive Control
    All animal cell lines

    其他说明

    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
    Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    产品性质

    质量水平100
    生物来源mouse
    抗体形式ascites fluid
    antibody product typeprimary antibodies
    克隆JLA20, monoclonal
    形式liquid
    包含≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)
    species reactivity (predicted by homology)all
    manufacturer/tradenameCalbiochem®
    储存条件OK to freeze
    同位素/亚型IgM
    运输wet ice
    储存温度−20℃

    安全信息

    储存分类代码11 - Combustible Solids
    WGKWGK 1
    闪点(F)Not applicable
    闪点(C)Not applicable

    Sigma-Aldrich

    推荐产品