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位置:首页 > 品牌 > Sigma-Aldrich > Transcreener® GDP TR-FRET Assay

Transcreener® GDP TR-FRET Assay

品牌
Sigma-Aldrich
货号
3021
包装型号
规格纯度
参考价格
13214.2 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Transcreener® GDP TR-FRET Assay
产品介绍:

产品说明

一般描述

The Transcreener® GDP TR-FRET Red Assay is a competitive immunoassay for GDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, regardless of what other substrates are used. Examples of enzymes include GTPases and fucosyltransferases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® GDP TR-FRET Red Assay provides the following benefits:

  • Accommodates GTP concentrations ranging from 0.1 μM to 1,000 μM.
  • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

数量

3021-1K = 1,000 assay, 384-well
3021-10K = 10,000 assay, 384-well

外形

Kit with buffered aqueous solutions

法律信息

Transcreener is a registered trademark of BellBrook Labs

基本信息

NACRESNA.84

产品性质

运输dry ice
储存温度−20℃

Sigma-Aldrich

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