产品说明
一般描述
Recombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (Kd = 5 x 10-14 M), plasmin (Kd = 2.3 x 10-10 M), and kallikrein (Kd = 1 x 10-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.
包装
25 mg in Glass bottle
1, 5 mg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
生化/生理作用
Product does not compete with ATP.
Reversible: yes
Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively
Primary Target
chymotrypsin
Cell permeable: no
警告
Toxicity: Standard Handling (A)
单位定义
One TIU (Trypsin Inhibitory Unit) is defined as the amount of aprotinin or product or inhibitor that will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1 µmol BAPNA per min at 25°C, pH 7.8.
外形
Contains no animal-derived components.
其他说明
Deutscher, M.P., 1990. Methods Enzymol.182, 83.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C284H432N84O79S7 |
分子量 | 6511.44 |
MDL编号 | MFCD00130541 |
EC索引号 | 2329949 |
产品性质
质量水平 | 200 |
重组 | expressed in Nicotiana |
测定 | ≥95% (SDS-PAGE) |
形式 | powder |
specific activity | >5 TIU/mg protein |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
颜色 | white to yellow |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich