产品说明

一般描述

codA基因编码胆碱氧化酶。

应用

西格玛公司生产的胆碱氧化酶已用于酶法测定牛奶中的胆碱，该方法采用了流动注射分析(FIA)系统和电位检测。†

生化/生理作用

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.
Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis helps to transform choline to glycinebetaine (N-trimethylglycine).

单位定义

在37 ℃条件下，当pH为8.0时，一个单位每分钟将形成1 μ摩尔的H₂O₂，其中1 μ摩尔胆碱氧化成甜菜醛。注：在胆碱氧化酶将胆碱转化为甜菜碱的过程中，为每μ摩尔的胆碱生成2 μ摩尔的H₂O₂。

制备说明

建议在补充10mM乙二胺四乙酸的pH 7.5磷酸盐缓冲液中重建胆碱氧化酶。重组材料可在2-8℃条件下储存至少2周。

基本信息

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H334 - H412
预防措施声明	P261 - P273 - P284 - P304 + P340 + P312 - P501
危险分类	Aquatic Chronic 3 - Resp. Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable