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Lambda DNA Mixed Digest

品牌
Sigma-Aldrich
货号
D2916
规格纯度
for DNA electrophoresis
参考价格
824.61 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Lambda DNA Mixed Digest
产品介绍:

产品说明

一般描述

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

应用

Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

组分

10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA

制备说明

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

其他说明

For optimal resolution, the recommended agarose gel concentration is 0.4%.

基本信息

NACRESNA.25

产品性质

质量水平200
100
形式buffered aqueous solution
用途100 uses (0.1 mg size)
20 uses (20 μg size)
适用性suitable for electrophoresis (DNA)
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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