产品说明
细胞系来源
Rat exocrine pancreatic tumour
细胞系描述
Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
应用
AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex
其他说明
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产品性质
生物来源 | rat pancreas |
生长模式 | Semi-adherent aggregates |
核型 | Not specified |
形态学 | Pancreas cells |
产品 | Amylase and exocrine enzymes |
受体 | Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich