RTG-2

产品说明

细胞系来源

Trout Rainbow gonad tissue

细胞系描述

From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24℃. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).

应用

Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Passage using 0.25% trypsin below 24℃. Cells may be seeded as few as 3x10,000 cells/ml 5% CO₂; 24℃. Fish cell lines detach easily during transit if the culture is very youn

其他说明

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产品性质