产品说明
细胞系来源
Mouse C57BL/1CRF lung carcinoma
细胞系描述
Derived from an alveologenic lung carcinoma from a C57BL/1CRF mouse. Can be maintained in a completely defined serum free medium, but the growth rate is much slower than in supplemented serum. Used in studies of lung metastases.
应用
Metastasis studies
培养基
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
产品性质
生物来源 | mouse lung |
生长模式 | Adherent |
核型 | Modal no. 85 |
形态学 | Epithelial |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich