产品说明
应用
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
包装
100 units in serum bottle
Package size based on DEA units
生化/生理作用
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
单位定义
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 ℃.
外形
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
分析说明
Protein determined by biuret.
基本信息
| 酶学委员会(EC)编号 | 3.1.3.1 ( BRENDA | IUBMB ) |
| EC 号 | 232-631-4 |
| MDL编号 | MFCD00131849 |
| eCl@ss | 42010105 |
产品性质
| 形式 | lyophilized powder |
| 质量水平 | 200 |
| specific activity | ≥100 DEA units/mg protein |
| 组成 | Protein, ≥50% |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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