WI 38VA13 Subline 2RA

产品说明

细胞系来源

Human Caucasian foetal lung, SV40 transformed

细胞系描述

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

应用

Transformation and virus studies: SV40

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质