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Glutamic-Oxalacetic Transaminase from porcine heart

谷氨酸-草酰乙酸转氨酶 来源于猪心脏

品牌
Sigma-Aldrich
CAS
9000-97-9
货号
G2751
规格纯度
Type I, ammonium sulfate suspension, 200-500 units/mg protein
参考价格
1482.56 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
9000-97-9
Glutamic-Oxalacetic Transaminase from porcine heart
L-Aspartate:2-oxoglutarate aminotransferase
Aspartate aminotransferase
GOT
谷氨酸-草酰乙酸转氨酶 来源于猪心脏
L-天冬氨酸:2-酮戊二酸氨基转移酶
天冬氨酸氨基转移酶
产品介绍:

产品说明

一般描述

Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

应用

Glutamic-Oxalacetic Transaminase from porcine heart has been used:

  • in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity
  • in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
  • as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

包装

10000 units in serum bottle

生化/生理作用

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

单位定义

一个酶活性单位是指在pH 7.5、37 ℃下,在 L-天冬氨酸存在时,每分钟将1.0 μmole的α-酮戊二酸转化为L-谷氨酸所需的酶量。在25 ℃下,一个单位相当于约2,000 O.D.(Karmen)单位。

外形

含 0.05M 马来酸盐和 2.5mM α-酮戊二酸盐的 3.0M (NH4)2SO4 中的悬浮液,pH 6.0

分析说明

由缩二脲确定的蛋白质。

基本信息

酶学委员会(EC)编号2.6.1.1 ( BRENDA | IUBMB )
EC 号232-571-9
MDL编号MFCD00131191
NACRESNA.54

产品性质

质量水平200
类型Type I
形式ammonium sulfate suspension
specific activity200-500 units/mg protein
异质活性glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%
运输wet ice
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

Sigma-Aldrich

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