产品说明
应用
Interleukin-12 (IL-12) human has been used to study IL-12 mediated enhancement of IL-12Rβ2 (IL-12 receptor) expression.
生化/生理作用
Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.
外形
lyophilized from a 0.2 μm filtered solution of PBS
分析说明
The specific activity was determined by the dose-dependent release of IFN-γ from the human NK92 cell line in presence of 20 ng/mL rIL-2.
法律信息
HumanKine is a registered trademark of Proteintech Group, Inc.
基本信息
| MDL编号 | MFCD00212975 |
| NACRES | NA.77 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human |
| 重组 | expressed in HEK 293 cells |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | ≤2 ng/mL EC50 |
| 分子量 | dimer 57 kDa (glycosylated) |
| 包装 | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
