产品说明
应用
HiCrome™ 麦康凯山梨醇琼脂建议用于选择性分离食物和动物饲料中的大肠杆菌 0157:H7。本品按照 Rappaport 和 Henigh 法配制。该培养基含山梨醇而不含乳糖，建议用于检测 大肠杆菌 0157:H7 的肠致病性菌株，这些菌株可发酵乳糖但不发酵山梨醇，因而会产生无色的菌落。大肠杆菌的山梨醇发酵菌株则产生粉红色菌落。变为红色的原因是：山梨醇生成酸、吸收中性红以及随后当培养基 pH 值低于 6.8 时染料发生颜色变化。大肠杆菌 0157:H7 被认为是出血性结肠炎的起因。
制备说明
Suspend 50.1g in 1 litre distilled water. Boil gently to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50℃. Mix well and pour into sterile petri plates. If desired add Tellurite-Cefixime Supplement to the molten and cooled medium (50℃) before pouring into sterile petri plates.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| MDL编号 | MFCD00240806 |
产品性质
| 质量水平 | 200 |
| 无菌性 | non-sterile |
| 形式 | powder |
| 保质期 | limited shelf life, expiry date on the label |
| 组成 | agar, 13.5 g/L B.C. indicator, 0.1 g/L bile salts mixture, 1.5 g/L casein enzymic hydrolysate, 17 g/L crystal violet, 0.001 g/L neutral red, 0.03 g/L proteose peptone, 3 g/L sodium chloride, 5 g/L sorbitol, 10 g/L |
| manufacturer/tradename | NutriSelect® Plus |
| 最终pH | 7.1±0.3 (25 ℃) |
| application(s) | agriculture environmental food and beverages microbiology |
| 适用性 | selective and differential for Escherichia coli selective and differential for coliforms selective and differential for enterobacteriaceae selective and differential for enterovirulent E. coli (EEC) |
安全信息
| 危险声明 | H412 |
| 预防措施声明 | P273 - P501 |
| 危险分类 | Aquatic Chronic 3 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
