产品说明
一般描述
The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Human Fas Ligand
应用
请参考Protocol了解详情。
生化/生理作用
Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
基本信息
NACRES | NA.32 |
产品性质
species reactivity | human |
包装 | kit of 96 wells (12 strips x 8 wells) |
technique(s) | ELISA: suitable capture ELISA: suitable |
input | sample type plasma sample type cell culture supernatant(s) sample type serum |
assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.37-1000 pg/mL |
detection method | colorimetric |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | human ... FASLG(356) |
组份列表
组份可单独销售
货号 | 组份 |
RABELADA | ELISA 1X Assay/Sample Diluent Buffer A (Item D1) |
RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
RABSTOP3 | ELISA Stop Solution (Item I) |
RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H290 |
预防措施声明 | P234 - P390 |
危险分类 | Met. Corr. 1 |
储存分类代码 | 8A - Combustible, corrosive hazardous materials |
WGK | WGK 3 |
Sigma-Aldrich