产品说明
一般描述
TRA-1-60 is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a neuraminidase sensitive epitope of a high molecular weight glycoprotein that are down-regulated upon differentiation. Recently this antigen has been proposed to be a form of the protein podocalyxin. TRA-1-60, can be detected in the serum of germ cell tumor patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
特异性
This antibody recognizes human TRA-1-60.
免疫原
Human embryonal carcinoma cell line 2102Ep.
应用
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
Anti-TRA-1-60 Antibody, clone TRA-1-60, Cy3 conjugate is an antibody against TRA-1-60 for use in ICC.
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-60 in mouse embryonic stem cells (SCR012).
质量
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.
目标描述
235/410 kDa calculated
外形
Ammonium sulfate precipitation followed by size exclusion.
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
储存及稳定性
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
H9 human embryonic stem cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
偶联物 | CY3 conjugate |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | TRA-1-60, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable |
同位素/亚型 | IgM |
NCBI登记号 | NP_001018121.1 |
UniProt登记号 | O00592 |
运输 | wet ice |
Gene Information | human ... PODXL(5420) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich