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Pellet Paint® NF Co-Precipitant

品牌
Millipore
货号
70748
规格纯度
Non-fluorescent visible DNA co-precipitant for automated sequencing applications
参考价格
10934.6 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Pellet Paint® NF Co-Precipitant
产品介绍:

产品说明

一般描述

Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending


Pellet Paint NF Co-Precipitant is anonfluorescent dye-labeled carrier compatiblewith fluorescent sequencing. It facilitatesrapid removal of BigDye Terminators duringthe alcohol precipitation of cycle sequencingreaction products. Cycle sequencing reactionscan be precipitated rapidly with 1 µl of carrierper reaction and centrifugation times of 10minutes. The easily visualized carrier providesa simple confirmation that precipitationhas occurred. Sequencing reaction productsare efficiently pelleted and dye-labeledterminators remain in the supernatant duringalcohol precipitation using the standardApplied Biosystems precipitation protocols.Resuspension of pelleted sequencing reactionproducts in deionized formamide can beconfirmed by checking for dissolution ofthe carrier. Pellet Paint NF Co-Precipitantis fully compatible with the ABI PRISM®BigDye Terminator Cycle Sequencing ReadyReaction. To avoid extra sample handling,Pellet Paint NF Co-Precipitant can be addeddirectly to the reaction mix, template DNA,crude PCR samples, or dilution buffer beforethe cycle sequencing reaction. Although PelletPaint NF absorbs in the UV range, accuratespectrophotometric measurements of DNA orRNA samples are possible; the absorbance ratio(provided with each package of Pellet PaintNF) can be used as a correction factor whendetermining nucleic acid concentration. PelletPaint NF Co-Precipitant has no detectableeffect on the sequencing reaction or sequenceaccuracy. Pellet Paint NF Co-Precipitant isa useful substitute for the original PelletPaint Co-Precipitant in applications wherefluorescent detection is used.

包装

1000, 125 reactions in Plastic ampoule

特点和优势

  • Efficient and rapid precipitation of BigDye® cycle sequencing products
  • Efficient removal of dye terminators
  • Direct visualization and tracking of precipitated material
  • No effect on sequencing reaction
  • Substitute for original Pellet Paint Co-Precipitant for fluorescent detection applications

组分

•: 125 µl or 1 ml: Pellet Paint NF Co-Precipitant


•: 1 ml or 8 ml: 3 M Sodium Acetate, pH 5.2

警告

Toxicity: Standard Handling (A)

法律信息

This product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates.
BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PELLET PAINT is a registered trademark of Merck KGaA, Darmstadt, Germany
Prism is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

基本信息

NACRESNA.85

产品性质

质量水平400
形式liquid
manufacturer/tradenameNovagen®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
运输ambient
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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