产品说明
一般描述
B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
特异性
This antibody recognizes the extracellular domain of B7-H3 (CD276).
免疫原
Epitope: Extracellular Domain
Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.
应用
Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.
Research Sub Category
Immunoglobulins & Immunology
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008).
Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).
Research Category
Inflammation & Immunology
质量
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.
目标描述
~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.
外形
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human placenta tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | rat |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | MJ8, monoclonal |
species reactivity | human |
species reactivity (predicted by homology) | mouse (based on 100% sequence homology) |
technique(s) | flow cytometry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2bκ |
NCBI登记号 | NP_598744.1 |
UniProt登记号 | Q8VE98 |
运输 | wet ice |
Gene Information | human ... CD276(80381) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich