产品说明
一般描述
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2N22 is a Rabbit recombinant monoclonal antibody that specifically detects IKKb. It targets an epitope within 24 amino acids from the N-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human IKKb.
应用
Quality Control Testing
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IKKβ in A549 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IKKβ in HepG2, L6, and NIH3T3 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound IKKb with a KD of 7.9 x 10-8 in an affinity binding assay.
Enzyme Immunoassay (ELISA) Analysis: Various dilutions of this antibody detected IKKβ in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Inhibitor of nuclear factor kappa-B kinase subunit beta (UniProt: O14920; also known as EC:2.7.11.10, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, Serine/threonine protein kinase IKBKB) is encoded by the IKBKB (also known as IKKB) gene (Gene ID: 3551) in human. The IKK complex is composed of three subunits, IKKa , IKKb, and IKKg (NEMO). While IKKa and IKKb are highly similar catalytic subunits and can phosphorylate IkB, IKKg is the regulatory subunit. IKKb is a serine kinase that plays an essential role in the NF-kB signaling pathway. It acts as a part of the canonical IKK complex in the conventional pathway of NF-kB activation. Its protein kinase domain is localized in amino acids 15-300. It phosphorylates inhibitors of NF-kB (IkB) on two critical serine residues that allow polyubiquitination of these inhibitors and their subsequent degradation by the proteasome. This allows free NF-kB to translocate into the nucleus and activate the transcription of genes involved in immune response, growth control, and protection against apoptosis. Upon cytokine stimulation, IKKb is phosphorylated on serine 177 and serine 181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and protein kinase Cz that enhances its activity. Once activated, it undergoes autophosphorylation on the C-terminal serine cluster, which reduces its activity and prevents prolonged activation of the inflammatory response. Mutations in IKBKB gene are known to cause life threatening immunodeficiency that develops in in infancy and increases susceptibility to bacterial, fungal, and viral infections. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, ZW., et al. (1999). J. Exp. Med. 189(11); 1839-1845).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
质量水平 | 200 |
生物来源 | rabbit |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 2N22, recombinant monoclonal |
描述 | recombinant, expressed in HEK 293 cells |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 86.56 kDa observed mol wt ~90 kDa |
纯化方式 | using Protein A |
species reactivity | mouse, human, rat |
species reactivity (predicted by homology) | bovine, monkey |
包装 | antibody small pack of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | ELISA: suitable affinity binding assay: suitable western blot: suitable |
同位素/亚型 | IgG |
表位序列 | N-terminal half |
Protein ID登记号 | NP_001177649 |
UniProt登记号 | O14920 |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich