Anti-SUPV3L1 Antibody, clone 2F5 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2F5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects ATP-dependent RNA helicase SUPV3L1 mitochondrial. It targets an epitope within 27 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of human ATP-dependent RNA helicase SUPV3L1, mitochondrial (SUPV3L1).

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SUPV3L1 in K562 and PC3 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SUPV3L1 in PC3 cells.
Affinity Binding Assay: A representative lot of this antibody bound SUPV3L1 peptides with a KD of 1.9 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SUPV3L1 in human small intestine and human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SUPV3L1, clone 2F5 ZooMAb, Cat. No. ZRB1065, is a recombinant Rabbit monoclonal antibody that specifically targets SUPV3L1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

ATP-dependent RNA helicase SUPV3L1, mitochondrial (UniProt: Q8IYB8; also known as EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3-like protein 1, SUPV3L1) is encoded by the SUPV3L1 (also known as SUV3) gene (Gene ID: 6832) in human. SUPV3L1 is a homodimeric protein in its free, but also exists as a component of the mitochondrial degradosome (mtEXO) complex that is a heteropentamer containing two copies of SUPV3L1 and three copies of polyribonucleotide nucleotidyltransferase 1 (PNPT1). mtEXO degrades 3′ overhang double-stranded RNA with a 3′-to-5′ directionality in an ATP-dependent manner. SUPV3L1 is synthesized with a transit peptide (aa 1-22), which is subsequently cleaved off. It contains one helicase C-terminal domain (aa 353-518) and one helicase ATP binding domain (aa 194-334). SUPV3L1 serves as a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. It plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non-coding processing intermediates. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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