Anti-PEDF (SERPIN F1) Antibody, clone 2C16 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2C16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Pigment epithelium-derived factor (PEDF).

免疫原

GST/His-tagged recombinant fragment corresponding to 152 amino acids from the C-terminal half of human Pigment epithelium-derived factor (PEDF).

应用

Affinity Binding Assay: A representative lot of this antibody bound human recombinant PEDF with a KD of 8.1 x 10-9 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PEDF (SERPIN F1) in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PEDF (SERPIN F1), clone 2C16 ZooMAb, Cat. No. ZRB1501, is a recombinant Rabbit monoclonal antibody that specifically targets PEDF and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Pigment epithelium-derived factor (UniProt: P36955; also known as PEDF, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1) is encoded by the SERPINF1 (also known as PEDF) gene (Gene ID: 5176) in human. Serpin F1 is a monomeric secreted protein that is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off to produce the mature form. Serpin F1 is synthesized by multiple cells, including retinal pigment epithelium, fibroblasts, mammary epithelium, adipocytes, hepatocytes and osteoblasts. It is enriched in retinal pigment epithelial cells and blood plasma. It displays neurotrophic activity and induces extensive neuronal differentiation in retinoblastoma cells. Its N-terminal (aa 44-121) displays neurite outgrowth-inducing activity while the C-terminal exposed loop (aa 382-418) is reported to be essential for its serpin activity. Serpin F1 also serves as a potent inhibitor of angiogenesis. Serpin F1 contains three beta sheets, 8 10 alpha helices, and a C terminal reactive center loop. In the cell, serpin F1 undergoes phosphorylation at Ser227 by protein kinase A that increases its anti-angiogenic activity and extracellular serpin F1 can be phosphorylated on serine 24 and serine 114 by casein kinase 2 that promotes its neurotrophic activity. Mutations in SERPINF1 gene are shown to cause Osteogenesis imperfecta 6 that is characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

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