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Anti-CACNA2D1 Antibody, clone 1K4 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1652
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
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数量
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产品名称:
Anti-CACNA2D1 Antibody, clone 1K4 ZooMAb® Rabbit Monoclonal
Voltage-dependent calcium channel subunit alpha-2/delta-1;Voltage-gated calcium channel subunit alpha-2/delta-1
产品介绍:

产品说明

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1K4 is a Rabbit recombinant monoclonal antibody that specifically detects Voltage-dependent calcium channel subunit alpha-2/delta-1 (CACNA2D1). It targets an epitope within 22 amino acids from the N-terminal, extracellular domain.

免疫原

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal, extracellular domain of human Voltage-dependent calcium channel subunit alpha-2/delta-1 (CCNA2D1).

应用

Quality Control Testing
Evaluated by Immunocytochemistry in U-251 MG cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CACNA2D1 in U-251 MG cells.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CACNA2D1 in human skeletal muscle.
Affinity Binding Assay: A representative lot of this antibody bound CACNA2D1 with a KD of 7.9 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Voltage-dependent calcium channel subunit alpha-2/delta-1 (UniProt: P54289; also known as Voltage-gated calcium channel subunit alpha-2/delta-1, CACNA2D1) is encoded by the CACNA2D1 (also known as CACNL2A, CCHL2A, MHS3) gene (Gene ID: 781) in human. CACNA2D1 is a dimeric protein formed by disulfide-linked 2 and chains. It regulates calcium current density and activation/inactivation kinetics of the calcium channel and is shown to play an important role in excitation-contraction coupling. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-1073), a transmembrane domain (aa 1074-1094), and a short cytoplasmic tail (aa 1095-1103). It also has a MIDAS-like motif (aa 259-263), which is present in its VWFA domain (aa 253-430). The MIDAS-like motif is shown to bind divalent metal cations and is required to promote trafficking of the a-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch. Five different isoforms of CACNA2D1 have been described that are produced by alternative splicing. Isoform 1 is expressed in skeletal muscle, isoform 2 is expressed in the central nervous system, and isoforms 3, 4, and 5 are expressed in the aorta. Isoforms 2, 4, and 5 are also observed in neuroblastoma cells. Mice bearing point mutations in the CACNA2D1 gene display reduced response to pregabalin and an abnormal central nervous system synaptic transmission. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Vergult, S., et al. (2015). Eur. J. Hum. Gen. 23(5); 628-632; Brust, PF., et al. (1993). Neuropharmacology. 32(11); 1089-1102).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1K4, recombinant monoclonal
描述recombinant, expressed in HEK 293 cells
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 124.57 kDa
纯化方式using Protein A
species reactivityhuman
species reactivity (predicted by homology)bovine, monkey
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
同位素/亚型IgG
表位序列N-terminus
Protein ID登记号NP_000713
UniProt登记号P54289
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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