Anti-Aquaporin-9 Antibody, clone 1E4 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1E4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Aquaporin-9. It targets an epitope within 22 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Aquaporin-9.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aquaporin-9 in HepG2 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Aquaporin-9 in human liver tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aquaporin-9 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound Aquaporin-9 peptide with a KD of 8.0 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Aquaporin-9, clone 1E4 ZooMAb, Cat. No. ZRB1456, is a recombinant Rabbit monoclonal antibody that targets Aquaporin-9 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Aquaporin-9 (UniProt: O43315; also known AQP-9, Aquaglyceroporin-9, Small solute channel 1) is encoded by the AQP9 (also known as SSC1) gene (Gene ID: 366) in human. Aquaporins are a family of small transmembrane proteins that facilitate osmotically driven water transport. Aquaporin-9 is a multi-pass membrane protein that forms a water channel with a broad specificity. It also mediates passage of a wide variety of small, non-charged solutes including purines, and pyrimidines. It is highly expressed in peripheral leukocytes and its expression is also reported in liver, lung, and spleen. Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA; aa 84-86 and 216-218). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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