Anti-ATG8 Antibody, clone 2B11 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2B11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Drosophila ATG8. It targets an epitope within 16 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptides corresponding to 16 amino acids each from the N-terminal region of Drosophila ATG8a and ATG8b.

应用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ATG8 in DSL2 Drosophila celld treated with chloroquine (50 mM overnight).
Affinity Binding Assay: A representative lot of this antibody bound ATG8 peptide with a KD of 6.0 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-ATG8, clone 2B11 ZooMAb, Cat. No. ZRB1585, is a recombinant Rabbit monoclonal antibody that specifically targets ATG8 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

目标描述

Autophagy-related 8a, isoform A and (UniProt: Q9W2S2; also known as LD05816p) and Autophagy-related 8b, isoform A (UniProt: Q9VEG5; also known as Autophagy-related 8b, isoform B, GEO01803p1) are encoded by Atg8a (also known as ATG8, DrAtg8a, LC3) and Atg8b (also known as ATG8, Atg8B) genes (Gene ID: 32001 and 42132) in Drosophila Melanogaster. Atg8 is a highly conserved and ubiquitously expressed protein that is upregulated under various stress conditions. Atg8 genes of multicellular animals is divided into three subfamilies LC3, GABARAP, GATE16 (depending on the animal). LC3 is the mammalian ortholog of Drosophila Atg8. Atg8 It shares a strong structural similarity to ubiquitin, despite the lack of similarity in amino acid sequence. The ubiquitin core of the Atg8 consists of a four-stranded central beta sheet, where the two central strands are parallel to each other and the two outer strands are antiparallel to the central strands. Between these strands are two alpha helices with alpha3 between beta2 and beta3 and alpha4 between beta3 and beta4. In resting cells, the majority of Atg8 is in found in an unlipidated state (Atg8-I) and during autophagy it is conjugated with a lipid moiety (Atg8-II). The lipidated form is the one that is recruited to autophagosomal membranes. The level of Atg8-II is an indicator of autophagosome activation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shpilka, T., et al. (2011). Genome Biology. 12(7); 226).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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