Anti-Helicase MOV-10 Antibody, clone 3B8 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Helicase MOV-10. It targets an epitope within 20 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Helicase MOV-10.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Helicase MOV-10 in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Helicase MOV-10 in HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:10 dilution from a representative lot detected Helicase MOV-10 in human pancreas tissue sections.
Affinity Binding Assay:: A representative lot of this antibody bound Helicase MOV-10 peptide with a KD of 2.4 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Helicase MOV-10, clone 3B8 ZooMAb, Cat. No. ZRB1112, is a recombinant Rabbit monoclonal antibody that specifically targets Helicase MOV-10 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blott

目标描述

Helicase MOV-10 (UniProt: EC:3.6.4.13; also known as Armitage homolog, Moloney leukemia virus 10 protein) is encoded by the MOV10 (also known as KIAA1631) gene (Gene ID: 4343) in human. MOV-10 is an evolutionarily conserved member of the UPF1p family of SF-1 ATP-dependent RNA helicases that serves as a putative 5′ to 3′ RNA helicase and contributes to UPF1 mRNA target degradation by translocation along 3′ UTRs. It is shown to inhibit retrovirus replication and overexpression of MOV-10 is shown to severely impair infectivity of HIV-1 and other lentiviruses. It is required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). MOV-10 is also shown to be interferon-inducible RNA helicase and can exhibit antiviral activity independent of its helicase function, against several positive- and negative-strand RNA viruses by enhancing type -I interferon induction. MOV-10 is reported to severely restrict human LINE1 (L1), Alu, and SVA retrotransposons. It associates with the L1 ribonucleoprotein particle, along with other RNA helicases including DDX5, DHX9, DDX17, DDX21, and DDX39A. MOV-10 is co-enriched in cytoplasmic foci along with TUT4. In developing neurons, it is shown to localize both in nucleus and cytoplasm, however, in adults it is found only in the cytoplasmic. It is also required for embryonic viability and for normal central nervous system development and function. It is reported to play two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis and regulates cytoskeletal mRNAs to influence neurite outgrowth. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodier, JL., et al. (2012). PLoS Genet 8(10); e1002941; Cuevas RA., et al. (2016). J. Immunol. 196(9); 3877-3886).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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