Anti-Cytokeratin 19 Antibody, clone 1B12 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1B12 is a Rabbit recombinant monoclonal antibody that specifically detects human Cytokeratin-19. It targets an epitope within 20 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Cytokeratin-19.

应用

Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cytokeratin 19 in MCF-7 cell lysate.

Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cytokeratin 19 in A549 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Cytokeratin 19 with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cytokeratin 19 in MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cytokeratin 19 in human appendix tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Keratin, type I cytoskeletal 19 (UniProt: P08727; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the KRT19 gene (Gene ID: 3880) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central -helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 belongs to the family of intermediate filament (IF) family but differs from other IF proteins in lacking the C-terminal tail domain. It is a heterotetramer of two type I and two type II keratins. CK-19 is expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. It is also observed in sweat glands, mammary gland ductal and secretory cells, bile ducts, bladder urothelium, and gastrointestinal tract. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jain, R., et al. (2010). Appl. Immunochem. Mol. Morphol. 18(1); 9-15).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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