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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Phospho-HDAC7-(Ser486) Antibody, clone 3O11 ZooMAb® Rabbit Monoclonal

Anti-Phospho-HDAC7-(Ser486) Antibody, clone 3O11 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1548
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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产品名称:
Anti-Phospho-HDAC7-(Ser486) Antibody, clone 3O11 ZooMAb® Rabbit Monoclonal
Histone deacetylase 7;EC:3.5.1.98;HD7;Histone deacetylase 7A;HD7a
产品介绍:

产品说明

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 3O11 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects HDAC7 phosphorylated on serine 486.

免疫原

KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 486 from the internal region of human Histone deacetylase 7 (HDAC7).

应用

Quality Control Testing
Evaluated by Western Blotting with a construct containing MBP-tagged recombinant fragment of HDAC7 phosphorylated on serine 486.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected a construct containing MBP-tagged recombinant HDAC7 phosphorylated on serine 486 but did not react with non-phosphorylated construct (Phospho-HDAC7 construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine)
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-HDAC7-(Ser486) in human prostate cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Phospho-HDAC7-(Ser486) in HEK293 cells treated with epidermal growth factor (EGF; 50 ng/mL; 10 min.).
Peptide Inhibition Assay: Target band detection in a lysate from A431 was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Histone deacetylase 7 (UniProt: Q8WUI4; also known as EC:3.5.1.98, HD7, Histone deacetylase 7A, HD7a) is encoded by the HDAC7 (also known as HDAC7A) gene (Gene ID: 51564) in human. Histone acetylation and deacetylation play a major role in determining chromatin structure and function. These modifications are achieved by histone acetyltransferases (HATs) and histone deacetylases (HDACs). In the deacetylated state, specific basic amino acids in histones are positively charged and interact with DNA s negatively charged phosphate groups and with negatively charged patches on neighboring nucleosomes. This promotes chromatin condensation to form heterochromatin. On the other hand, acetylation neutralizes these positive charges and promoting the less condensed and more accessible chromatin conformation known as euchromatin. HDAC7 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). It shuttles between the nucleus and the cytoplasm and in the nucleus, it associates with distinct subnuclear dot-like structures. Its nuclear export signal is localized in amino acids 918-952. Its deacetylase activity maps to a C-terminal domain and is dependent on its interaction with other class I HDACs in the nucleus. HDAC7 can undergo phosphorylation by PKN1 and PKN2 that impairs its nuclear import. Its phosphorylation on serine 155 by MARK2, MARK3, and PRKD1 is shown to promote its interaction with 14-3-3 proteins and facilitate its export from the nucleus by binding to specific phosphoserine 486 on HDAC7. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3O11, recombinant monoclonal
描述recombinant, expressed in HEK 293 cells
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 102.93 kDa
observed mol wt ~42 kDa
纯化方式using Protein A
species reactivityhuman
species reactivity (predicted by homology)mouse, monkey, bovine, rat
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
表位序列Internal
Protein ID登记号NP_056216
UniProt登记号Q8WUI4
application(s)peptide synthesis
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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