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位置:首页 > 品牌 > Sigma-Aldrich > Anti-HNF-1β Antibody, clone 1M22 ZooMAb® Rabbit Monoclonal

Anti-HNF-1β Antibody, clone 1M22 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1306
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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数量
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产品名称:
Anti-HNF-1β Antibody, clone 1M22 ZooMAb® Rabbit Monoclonal
产品介绍:

产品说明

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1M22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Hepatocyte nuclear factor 1β . It targets an epitope within 24 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Hepatocyte nuclear factor 1-beta.

应用

Quality Control Testing
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HNF-1β in A549 cell lysate.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HNF-1β in HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HNF-1 β in human kidney tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound HNF-1β with a KD of 2.7 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HNF-1β, clone 1M22 ZooMAb®, Cat. No. ZRB1306, is a recombinant Rabbit monoclonal antibody that specifically targets HNF-1b and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Hepatocyte nuclear factor 1β (UniProt: P35680; also known as HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, TCF-2, Variant hepatic nuclear factor 1, vHNF1) is encoded by the HNF1B (also known as TCF2) gene (Gene ID: 6928) in human. HNF-1β is a transcription factor of the HNF1 homeobox family that binds to the inverted palindrome 5′-GTTAATNATTAAC-3′. It binds to DNA as a dimer. It controls endoderm development and is shown to be crucial for pancreatic and renal development. It is also involved in the hepatobiliary specification of hepatoblasts to cholangiocytes during liver development and is strongly expressed throughout adult biliary epithelium. Over-expression of HNF-1β is associated with pathological subtype of primary tumor and its expression in hepatocellular carcinoma is associated with the change of phenotype on recurrence. Mutations in HNF1B are known to cause Renal cysts and diabetes syndrome (RCAD), an autosomal dominant disorder comprising non-diabetic disease resulting from abnormal renal development. HNF1B knockout mice are shown to suffer from severe neonatal cholestasis and jaundice due to the abnormally developed gall bladder and dysfunctional intrahepatic bile ducts. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kolatsi-Joannou, M., et al. (2001). J. Am. Soc. Nephrol. 12(10); 2175-2180; Yu, D-D., et al. (2015). Sci. Rep. 5; Article 13319).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1M22, recombinant monoclonal
描述recombinant, expressed in HEK 293 cells
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 61.32 kDa
observed mol wt ~64 kDa
species reactivityhuman
包装antibody small pack of 25 μL
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
表位序列C-terminal half
Protein ID登记号NP_000449
UniProt登记号P35680
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

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