产品说明
一般描述
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.
应用
Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.
质量
Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.
外形
Protein A purified
其他说明
Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
偶联物 | ALEXA FLUOR® 555 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | NE14, monoclonal |
species reactivity | rat, mouse, pig |
species reactivity (predicted by homology) | human (100% sequence homology), porcine (immunogen homology) |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NP_066554 |
UniProt登记号 | P12036 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich