产品说明
一般描述
A specific substrate for pancreatic elastase (km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase, Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.
A specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.
Suggested protocol: see Largman, C. (1983)
包装
5 mg in Plastic ampoule
生化/生理作用
km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
Product does not compete with ATP.
Primary Target
A specific substrate for pancreatic elastase
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
外形
45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
重悬
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
其他说明
Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C25H34N6O9 |
分子量 | 562.57 |
产品性质
质量水平 | 100 |
测定 | ≥95% (HPLC) |
形式 | lyophilized solid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles desiccated |
颜色 | white to off-white |
溶解性 | ethanol: 1 mg/mL water: soluble |
运输 | ambient |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich