产品说明
应用
Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.
生化/生理作用
Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.
单位定义
One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.
外形
Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.
基本信息
| 酶学委员会(EC)编号 | 2.7.11.12 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD03458230 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | ≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM)) |
| 分子量 | 76 kDa (monomer) |
| UniProt登记号 | Q13976 |
| 相关疾病 | cancer |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PRKG1(5592) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
