产品说明
应用
Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 ℃ using a final concentration of 50 μM [32P] ATP.
生化/生理作用
SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na+/K+-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na+ channel ENaC activity.
单位定义
One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 ℃ and pH 7.2 using a final concentration of 50 μM [32P] ATP.
外形
Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.
基本信息
| MDL编号 | MFCD07371188 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 测定 | ≥75% (SDS-PAGE) |
| 形式 | aqueous glycerol solution |
| specific activity | 100 units/mg protein (Bradford) |
| 分子量 | protein apparent mol wt ~66 kDa |
| UniProt登记号 | Q9HBY8 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... SGK2(10110) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
