产品说明
一般描述
Clozapine N-oxide hydrochloride is a water soluble form of Clozapine N-oxide (CNO) (Sigma cat# C0832). CNO is a pharmacologically inert metabolite of the atypical anti-psychotic drug clozapine. CNO is a ligand for the engineered Gq protein coupled receptors (GPCRs) in the chemogenetic Designer Receptors Exclusively Activated by Designer Drug (DREADD). The typical use of DREADD is testing, enhancing neuronal firing and activating Gq signaling in neuronal and non-neuronal cells.
The selective targeting of DREADD systems to cell population can be achieved by using a cell type-specific promoter to drive DREADD expression, and the expression of this promoter can be further controlled using a recombinase-based system. DREADDs possess a low affinity for endogenous ligands and little constitutive activity, but may be activated by synthetic compounds such as CNO.
DREADD systems are activated by low nM concentrations of CNO and mobilize intracellular calcium. CNO appears to be pharmacologically and behaviorally inert in mice and rats when administered at the recommended doses (generally 0.1–3 mg/kg). CNO is commonly administrated via injection but also can be mixed into food chow or drinking water.
其他说明
High concentration Solutions of (40mg/ml) can be prepared from the water soluble CNO and be used in CNO applications without the use of DMSO.
基本信息
| 经验(实验)分子式 | C18H19ClN4O · xHCl |
| 分子量 | 342.82 (free base basis) |
| NACRES | NA.77 |
产品性质
| 生物来源 | synthetic |
| 测定 | ≥98% (HPLC) |
| 形式 | powder |
| 储存条件 | desiccated |
| 溶解性 | DMSO: 40 mg/mL water: 40 mg/mL |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
| SMILES string | ClC1=CC2=C(NC(C=CC=C3)=C3C(N4CC[N+]([O-])(C)CC4)=N2)C=C1.[xHCl] |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H301 - H336 |
| 预防措施声明 | P301 + P310 + P330 |
| 危险分类 | Acute Tox. 3 Oral - STOT SE 3 |
| 靶器官 | Central nervous system |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
