产品说明
一般描述
v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.
特异性
Reactive with human c-erB-4/Her 4 cytoplasmic domain of the protein (residues 1250-1264), a member of the EGF receptor (type 1) family involved in cell growth regulation. Expression or function may be altered in human cancers.
免疫原
Synthetic peptide sequence RSTLQHPDYLQEYST from the cytoplasmic domain of the protein, residues 1250-1264.
Epitope: Cytoplasmic domain of the protein, residues 1250-1264.
应用
Detect erbB4 using this Anti-erbB4 Antibody, clone HFR1/2G4 validated for use in WB, IP, IH(P).
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: Effective for formalin-fixed, paraffin-embedded sections.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of erbB-4/HER-4 Monoclonal Antibody: DCIS and Pancreatic Cancer
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
Apoptosis & Cancer
质量
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected C-ERB-4 on 10 μg of A431 lysates.
目标描述
~147 kDa
联系
Replaces: 04-348
外形
Purified mouse monoclonal IgM in 0.02 M PB with 0.25 M NaCl, pH 7.6, 0.01% Sodium Azide.
Purified
Format: Purified
储存及稳定性
Stable refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
分析说明
Control
A431 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | HFR1/2G4, monoclonal |
species reactivity | human, rat |
species reactivity (predicted by homology) | mouse |
technique(s) | immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1, kappa |
NCBI登记号 | NP_005226.1 |
UniProt登记号 | Q15303 |
运输 | wet ice |
Gene Information | human ... ERBB4(2066) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich