Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

产品说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

特异性

Broad species cross-reactivity expected due to sequence homology.
Recognizes Histone H3, Mr 17 kDa

免疫原

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.

应用

Chromatin Immunoprecipitation:
2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Use Anti-Histone H3 Antibody, CT, pan, clone A3S (Rabbit Monoclonal Antibody) validated in ChIP, WB to detect Histone H3 also known as Histone H3.

质量

Routinely evaluated by western blot analysis.
Western Blot Analysis:
1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

目标描述

17 kDa

外形

Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

分析说明

Control
Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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