Anti-Myb Antibody, clone 1-1

产品说明

一般描述

Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5′-YAAC[GT]G-3′. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

特异性

This antibody recognizes Myb, Mr 80 kDa, containing C-terminal amino acids from 401-636 (Salomoni, P., 1997).

免疫原

Recombinant murine Myb purified by SDS-PAGE (Nicot, C., 2001) Clone 1-1.

应用

Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.
Immunohistochemistry:
Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.

质量

Routinely evaluated on RIPA lysate from Jurkat cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.

目标描述

80 kDa

外形

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.
Format: Purified
Ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography.

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Jurkat cell lysate.
Included Positive Antigen Control: Catalog # 12-303, Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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