Anti-p300 CT Antibody, clone RW128

产品说明

一般描述

p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes.
p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
p300 and CBP are thought to increase gene expression in three ways:
(1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
(2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
(3) by acting as adaptor molecules.

特异性

Recognizes p300 and co-immunoprecipitates E1A.

免疫原

Epitope: C-terminus
GST-fusion to the C-Terminus of human p300

应用

Research Category
Epigenetics & Nuclear Function
Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.
Research Sub Category
Histones

Chromatin Biology

RNA Binding Protein (RBP)

质量

routinely evaluated by immunoblot on HeLa nuclear extract

目标描述

300 kDa

外形

Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol
Protein G Purified

储存及稳定性

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Positive Control Included: HeLa Nuclear Extract (12-309)

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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