Anti-Rho (-A Antibody, -B, -C), clone 55

产品说明

一般描述

Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking.

特异性

Recognizes Rho (-A, -B, -C), Mr 22 kDa.

免疫原

Recombinant human RhoA containing amino acids 1-155. Clone 55.

应用

Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.
Research Sub Category
Cytoskeletal Signaling
Research Category
Signaling
Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB.

质量

Routinely evaluated by western blot on RIPA lysates from Jurkat or HL-60 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.

目标描述

22 kDa

外形

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing BSA containing 0.09% sodium azide and 50% glycerol.
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

分析说明

Control
HL-60 cell lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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