产品说明
应用
Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 ℃ using a final concentration of 50 μM [32P] ATP.
生化/生理作用
Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.
单位定义
One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP
外形
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
基本信息
| MDL编号 | MFCD07370972 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 重组 | expressed in E. coli |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| enzyme activity | ≥10 units/mg protein |
| 分子量 | protein apparent mol wt ~58 kDa |
| UniProt登记号 | Q9HC98 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... NEK6(10783) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
